3502 type a genome sequence (ATCC)
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3502 type a genome sequence
3502 Type A Genome Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3502+genome+sequence/HCT116-SLC35F2-KO-c2/pmc11310924-143-21-20
Average 96 stars, based on 335 article reviews
3502 Type A Genome Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3502+genome+sequence/HCT116-SLC35F2-KO-c2/pmc11310924-143-21-20
Average 96 stars, based on 335 article reviews
3502 type a genome sequence - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "Exploring genomic diversity in Clostridium botulinum using DNA microarrays"
Article Title: Exploring genomic diversity in Clostridium botulinum using DNA microarrays
Journal: The botulinum journal
doi: 10.1504/tbj.2012.050195
Figure Legend Snippet: CGH results of outbreak-related type A1 strains Notes: The top track depicts each coding region sequence (CDS) in the C. botulinum strain ATCC 3502 genome sequence. Subsequent tracks depict log2 signal ratios of the reference strain (ATCC3502) compared to each test strain (indicated below each track). Regions whose presence varies among outbreak-pairs are circled and their genomic location relative to ATCC 3502 genome is depicted by a letter above the top track. Region A corresponds to a ferrichrome uptake system, region B corresponds to a restriction/modification system, and region C corresponds to a glycosyltransferase.
Techniques Used: Sequencing, Modification
Figure Legend Snippet: Scheme used to select probes for the Group I subtyping microarray Notes: A total of 225 probes were selected. 146 probes corresponding to strain variable regions of the ATCC 3502 genome sequence were selected based on CGH analysis using Group I types A, B, and F test strains. In silico analysis was used to select the remaining probes representing genes not present in ATCC 3502, specific for either OkraB or LanglelandF strains, or other specific genes of interest.
Techniques Used: Microarray, Sequencing, In Silico
Related Articles
Polymerase Chain Reaction:Article Title: Phylogenetic Analysis of Clostridium botulinum Type A by Multi-Locus Sequence Typing Article Snippet: Sequencing analysis was performed at the University of Wisconsin Biotechnology Center and final sequencing results were analyzed using the Vector NTI Suite Program (Invitrogen). .. Accession numbers for the resulting nucleotide sequences are as follows: rpoB ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372261-EU372269","start_term":"EU372261","end_term":"EU372269","start_term_id":"164612298","end_term_id":"164612314"}} EU372261-EU372269 ), recA ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372253-EU372260","start_term":"EU372253","end_term":"EU372260","start_term_id":"164612316","end_term_id":"164612330"}} EU372253-EU372260 ), oppB ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372242-EU372252","start_term":"EU372242","end_term":"EU372252","start_term_id":"164612332","end_term_id":"164612352"}} EU372242-EU372252 ), mdh ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372232-EU372241","start_term":"EU372232","end_term":"EU372241","start_term_id":"164612354","end_term_id":"164612372"}} EU372232-EU372241 ), hsp60 ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372223-EU372231","start_term":"EU372223","end_term":"EU372231","start_term_id":"164612400","end_term_id":"164612416"}} EU372223-EU372231 ), aceK ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372210-EU372222","start_term":"EU372210","end_term":"EU372222","start_term_id":"164612374","end_term_id":"164612398"}} EU372210-EU372222 ), and aroE ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372197-EU372209","start_term":"EU372197","end_term":"EU372209","start_term_id":"164612418","end_term_id":"164612442"}} EU372197-EU372209 ). table ft1 table-wrap mode=article t1 caption a4 Details on primer sequences and the PCR conditions of 7 housekeeping genes used in MLST analysis The C. botulinum Type A Sequencing:Article Title: Phylogenetic Analysis of Clostridium botulinum Type A by Multi-Locus Sequence Typing Article Snippet: Sequencing analysis was performed at the University of Wisconsin Biotechnology Center and final sequencing results were analyzed using the Vector NTI Suite Program (Invitrogen). .. Accession numbers for the resulting nucleotide sequences are as follows: rpoB ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372261-EU372269","start_term":"EU372261","end_term":"EU372269","start_term_id":"164612298","end_term_id":"164612314"}} EU372261-EU372269 ), recA ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372253-EU372260","start_term":"EU372253","end_term":"EU372260","start_term_id":"164612316","end_term_id":"164612330"}} EU372253-EU372260 ), oppB ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372242-EU372252","start_term":"EU372242","end_term":"EU372252","start_term_id":"164612332","end_term_id":"164612352"}} EU372242-EU372252 ), mdh ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372232-EU372241","start_term":"EU372232","end_term":"EU372241","start_term_id":"164612354","end_term_id":"164612372"}} EU372232-EU372241 ), hsp60 ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372223-EU372231","start_term":"EU372223","end_term":"EU372231","start_term_id":"164612400","end_term_id":"164612416"}} EU372223-EU372231 ), aceK ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372210-EU372222","start_term":"EU372210","end_term":"EU372222","start_term_id":"164612374","end_term_id":"164612398"}} EU372210-EU372222 ), and aroE ( {"type":"entrez-nucleotide-range","attrs":{"text":"EU372197-EU372209","start_term":"EU372197","end_term":"EU372209","start_term_id":"164612418","end_term_id":"164612442"}} EU372197-EU372209 ). table ft1 table-wrap mode=article t1 caption a4 Details on primer sequences and the PCR conditions of 7 housekeeping genes used in MLST analysis The C. botulinum Type A Article Title: Analysis of genomic differences among Clostridium botulinum type A1 strains Article Snippet: Sequence alignment showed that there was a synonymous single nucleotide polymorphism (SNP) in the region encoding the heavy chain between Allergan strain and ATCC 3502 and UMASS strains. .. Second, comparative genomic hybridization (CGH) demonstrated that the UMASS strain and a strain expected to be derived from ATCC 3502 in the Centers for Disease Control and Prevention (CDC) laboratory (ATCC 3502*) differed in gene content compared to the Article Title: Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray. Article Snippet: A focused oligonucleotide microarray featuring 62 probes targeting strain variable regions of the Clostridium botulinum strain ATCC 3502 genome sequence was developed to differentiate C. botulinum type A strains.. The strain variable regions were selected from deletions identified among a panel of 10 type A strains compared to the strain ATCC 3502 genome sequence using high density comparative genomic hybridization microarrays.. The focused microarray also featured specific probes for the detection of the neurotoxin genes of various serotypes (AeG), toxin gene cluster components (ha70 and orfX1), and fldB as a marker for proteolytic clostridia (Group I). Article Title: Genetic Homogeneity of Clostridium botulinum Type A1 Strains with Unique Toxin Gene Clusters Article Snippet: For the present study, the array features were based on the genome sequence of C. botulinum strain ATCC 3502. .. The ATCC 3502 strain used as a reference in our hybridization experiments displayed some minor changes compared to the Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 Article Snippet: .. The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published Article Title: Important Role of Class I Heat Shock GeneshrcAanddnaKin the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 Article Snippet: .. The primers for the six class I heat shock genes studied (hrcA, grpE, dnaK, dnaJ, groES, and groEL) and for the 16S rrn were designed based on the published Hybridization:Article Title: Analysis of genomic differences among Clostridium botulinum type A1 strains Article Snippet: Sequence alignment showed that there was a synonymous single nucleotide polymorphism (SNP) in the region encoding the heavy chain between Allergan strain and ATCC 3502 and UMASS strains. .. Second, comparative genomic hybridization (CGH) demonstrated that the UMASS strain and a strain expected to be derived from ATCC 3502 in the Centers for Disease Control and Prevention (CDC) laboratory (ATCC 3502*) differed in gene content compared to the Article Title: Genetic Homogeneity of Clostridium botulinum Type A1 Strains with Unique Toxin Gene Clusters Article Snippet: For the present study, the array features were based on the genome sequence of C. botulinum strain ATCC 3502. .. The ATCC 3502 strain used as a reference in our hybridization experiments displayed some minor changes compared to the Derivative Assay:Article Title: Analysis of genomic differences among Clostridium botulinum type A1 strains Article Snippet: Sequence alignment showed that there was a synonymous single nucleotide polymorphism (SNP) in the region encoding the heavy chain between Allergan strain and ATCC 3502 and UMASS strains. .. Second, comparative genomic hybridization (CGH) demonstrated that the UMASS strain and a strain expected to be derived from ATCC 3502 in the Centers for Disease Control and Prevention (CDC) laboratory (ATCC 3502*) differed in gene content compared to the Control:Article Title: Analysis of genomic differences among Clostridium botulinum type A1 strains Article Snippet: Sequence alignment showed that there was a synonymous single nucleotide polymorphism (SNP) in the region encoding the heavy chain between Allergan strain and ATCC 3502 and UMASS strains. .. Second, comparative genomic hybridization (CGH) demonstrated that the UMASS strain and a strain expected to be derived from ATCC 3502 in the Centers for Disease Control and Prevention (CDC) laboratory (ATCC 3502*) differed in gene content compared to the Plasmid Preparation:Article Title: Genetic Homogeneity of Clostridium botulinum Type A1 Strains with Unique Toxin Gene Clusters Article Snippet: For the present study, the array features were based on the genome sequence of C. botulinum strain ATCC 3502. .. The ATCC 3502 strain used as a reference in our hybridization experiments displayed some minor changes compared to the |